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The commonly used method is high-performance liquid chromatography, supplemented by other validation methods, as detailed below

ME/CFS patients exhibit dysregulation of the HPA axis [34]

Abdel GhfarSSAliMEMomenahMAAl-SaeedFAAl-DoaissAAMostafaYSet al

123126 Mutant mice lacking the AA transporter SVCT2 have low adrenal tissue catecholamine levels, 12 and AA deficiency in humans is associated with listlessness and decreased epinephrine response