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Bound mouse C1q was detected by polyclonal anti-mouse C1q serum from goat (Creative Biolabs, CTA-P-023) (diluted 1:2000 in PBS/T/S) followed by detection using an anti-goat IgG AP-conjugated antibody from rabbit (Merck)

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The mass spectrum of complex 2 treated with plasma for 1 min displayed the same ionization pattern with signals at m/z = 254 and at m/z = 376 corresponding to the [M L] + and [M + Na] + , as spectrum for the untreated complex

[65] indicated elevated blood homocysteine levels in individuals with Type 2 diabetes mellitus (T2DM), particularly among those with diabetic nephropathy (DN) and diabetic retinopathy (DR), but the precise role of homocysteine in the development of T2DM and its associated complications remains ambiguous