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Briefly, 2 g plasmid, 4 L P3000 and 4 L Lipofectamine 3000 were mixed into 500 L Opti-MEM medium in order for 30 min, then added up to 3 mL with DMEM medium before transferring to 6-well plate

doi: 10.1007/s11064-016-2074-y

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