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The adaptor ligation step was performed as directed albeit with a custom pre-annealed oligo adaptor comprising of YL001 (/5Phos/GATCGGAAGAG/3ddC/) annealed to one of YL002:005 (AGCGGCAATTTCACACAGGACAAGCAGAAGACGGCATACGAGATNNNNNNNN AGTG GTGACTGGAGTTCAGACGTGTGCTCTTCCGATC*T) where the 4-base underlined sequence serves as a variable barcode unique to each oligo

ALCAR functionally inhibits angiogenesis in vitro We have previously shown that ALCAR inhibits endothelial cells by regulating angiogenesis in hypoxic and inflammatory conditions in vitro and in vivo [30]

Immune checkpoint inhibitors: Review and management of endocrine adverse events

BMC Bioinformatics 5 , 113 (2004)

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