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Vascular damage in a mouse model of diabetic retinopathy: relation to neuronal and glial changes

I had a lipoma removed and was very pleased with the work

Circ Res 2000;87:349-54

Immunoprecipitation was then performed using the Catch and Release Immunoprecipitation Kit (EMD Millipore), according to the manufacturers instructions with 250 g of total protein from cells or 400 g from tissues, and 1 g of an anti-IGF-1R antibody (#9750, Cell Signaling)