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The absence of GR in Av

sought to test the effect of chronic 14-day valproate on two markers of neuroplasticity, myristoylated alanine-rich C kinase substrate (MARCKS) and GAP-43, both known PKC substrates

Extracted proteins were denatured in 100 L of 6 M urea, reduced with 10 mM dithiothreitol (DTT) at room temperature for 60 min, and alkylated with 15 mM iodoacetamide in the dark for 60 min

Supplementary Table 1), which was verified in the IGEN format (Fig