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Protoplasts in a solution of 1 mM D-luciferin (Biosynth AG), 5% fetal bovine serum (Sigma-Aldrich), 50 g/mL ampicillin, 140 mM NaCl, 115 mM CaCl2, 4.6 mM KCl, 1.86 mM MES pH 5.7 and 4.6 mM glucose were added to white, flat-bottomed 96-well plates (Lumitrac, Greiner Bio-one) at a concentration of 2 10 5 cells/mL

Unlike synthetic painkillers that merely block symptoms, BPC-157 actually rebuilds the structural foundation of healthy joints

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An insulin syringe is often the best choice for this purpose, as it has very fine markings that allow for precise measurements of small volumes

However, it's important to note that these are experimental settings and may not directly translate to human use