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The primary metabolite, phosphoramide mustard, is a nitrogen mustard molecule considered the most therapeutically effective metabolite owing to its significant ability to induce DNA damage by intercalating between the two strands (Vredenburg et al

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Analysis of vessel density and vessel branching were done using AngioTool version 0.6a (02.18.14), an open source windows executable software which provides automated measures of vessel area and number of junctions 42