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A 2 mL aliquot of the supernatant was transferred to a test tube, to which 2 mL of glacial acetic acid and 2 mL of 2.5% (w/v) acidic ninhydrin solution were added

Secondly, the persistently high level of LH secretion promotes ovarian stromal vascular proliferation, increased stromal blood flow, enhanced oxygen metabolism, and increased production of reactive oxygen species (ROS), triggering oxidative stress, which adversely affects oocyte maturation, fertilization, embryo development, and the progress of pregnancy (Li et al., 2022)

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A median liver triacylglycerol also reduced from 3.7%1.8% significantly in the CR group ( p = 0.004) post-intervention