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Microbial dysbiosis and biofilm formation in chronic wounds When the microbiomes equilibrium is disrupted (dysbiosis), opportunistic and pathogenic bacteria such as P aeruginosa and S

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The fresh sample was extracted from 10% TCA solution, centrifuged 10,000 g , for 10 min under cold condition and the supernatant was incubated in an assay mixture [0.2 M phosphate buffer, 15 mM dithiobis-(2-nitrobenzoic acid)] and diluted with water for 30 min

PMID 7947656