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All cells except for hNF-1 were grown and maintained as monolayer cultures at 37 C in 5% CO 2 in a humidified atmosphere, using DMEM (high glucose with l-glutamine and phenol red, Fujifilm Wako Pure chemical corporation), supplemented with penicillinstreptomycin solution (100) (Fujifilm Wako Pure Chemical Corporation), and 10 vol% FBS (Sigma-Aldrich Co., Llc.)

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Retatrutide adds glucagon receptor activation on top of those same two targets