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Detection of mitochondrial ROS, apoptosis, and viability For mitochondrial ROS measurements, cells were stained with MitoSOX Red (2.5 M, 30 minutes, dark), washed with phosphate-buffered saline (PBS), detached using trypsin-EDTA, and washed twice in Hanks Balanced Salt Solution (HBSS) by centrifugation (5 min, 1200 rpm, room temperature)

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However, the stability and reproducibility of metabolomics biomarkers in different populations, as well as interactions between metabolites and other biological factors need to be further clarified

Neuroimage 197 , 502511 (2019)

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