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Description

Samples were de-paraffinized and antigens unmasked by heating in a pressure cooker to 95C and 15 psi for 20 min in Trilogy alkaline solution with EDTA (Cell Marque), followed by 3 min in 90% formic acid

Glutathione is supplied as a powder in 600 mg and 1500 mg sizes

Additional analysis of differential scMEP marker expression between the CD1c hi (quantile 4) vs CD1c low (quantile 1) cells confirmed that inducible factors GLUT1, MCT1 and PFKFB4 are significantly elevated in CD1c hi populations (Fig