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For quantitative reverse transcription PCR (RTqPCR), 1,000 ng of total RNA was reverse transcribed into complementary DNA (cDNA) using a Hifair II 1st Strand cDNA Synthesis Kit (Yeasen, China) following the manufacturers instructions

Yimam KK, Bowlus CL

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Daz-Blancas V, Medina DI, Padilla-Ortega E, Bortolini-Zavala R, Melissa OR, Luna-Brcenas G