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Clinical studies demonstrate that GHK-Cu: Improves skin elasticity Reduces fine lines in some cosmetic studies Increases dermal thickness Enhances wound healing in limited models However: Most trials are small Many are cosmetic rather than mechanistic Long-term systemic data are limited Claims regarding hair regrowth and systemic anti aging effects exceed what controlled human trials demonstrate

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After rewarming on the next day, the slides were co-incubated with the secondary antibody (1:200 dilutions), diluted in PBS for 1 h (protected from light), and the excess antibody was washed with PBS

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