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Killed mice were then perfused transcardially with 50 ml of cold phosphate buffered saline (PBS), the meninges were removed, and the brain was blotted dry

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Dopamine plays a crucial role in modulating the reward network in chronic insomnia disorder (CID), particularly in the context of comorbid depression, where genetic variations related to DA can differentially affect functional connectivity within the nucleus accumbens and broader reward networks

New York: Springer