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Bacterial co-cultures with human colonic organoid-derived monolayers To facilitate access to the apical side of the intestinal epithelium for bacterial co-cultures, organoids were cultured as 2D monolayers as follows, using a protocol adapted from Gonyar et al

Studies have reported that 6 mA is implicated in regulation transcription, transposon activity, and other functions ( Liu et al., 2023 )