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For immunofluorescence (IFA) analysis, deparaffinized colon sections were blocked with 10% normal goat serum for 10 min at room temperature

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PhAN9 (A12M): PhAN9 _A12M_fw, PhAN9 _A12M_rv, PhAN9 (C13S): PhAN9 _C13S_fw, PhAN9 _C13S_rv, PhAN9 (N108H): PhAN9 _N108H_fw, PhAN9 _N108H_rv (Supplementary Table 4) using pANT15 as template

IGF-1/PI3K/Akt signaling stimulates the rate of protein synthesis via p70S6Kinase and p90 ribosomal S6 kinase and negatively regulates protein degradation, predominantly by its inhibiting effect on proteasomal and lysosomal protein degradation