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Curr Protoc Immunol (2010) Chapter 19:Unit19.13.10.1002/0471142735.im1913s91 48 WangHYangHTraceyK

Donnelly, D

The cells were pretreated with caffeine (256 or 512 M) for 1 h and subsequently irradiated with UVB lamp for 5 min, then lysed by adding KDalert lysis buffer from the kit, and finally immunoprecipitated with SIRT3 antibody