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Betaine feeding prevents the blood alcohol cycle in rats fed alcohol continuously for 1 month using the rat intragastric tube feeding model

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Madsen RK, Lundstedt T, Gabrielsson J, Sennbro CJ, Alenius GM, Moritz T et al

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The precipitates were washed thrice with high-salt buffer (PIP lysis buffer adjusted to 300 mM NaCl), boiled with SDS loading buffer, and subjected to SDS-PAGE