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5 Other BBB-related neurological disorders The BBB is associated not only with epilepsy but also with many other neurological diseases

Clarified supernatant was kept after spinning at 13.2 rpm for 15 min, and a total of 40 l was taken to determine the expression of endogenous protein

Given the limitations of patient derived cell models, to further test the translational impact of our findings we applied our recently reported approach of treating acute slice cultures of human glioma surgical specimens with drugs and deconvolving cell type-specific responses with scRNA-seq (Zhao et al, 2021)