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For the B 12 bolus experiment, mice were administered 5 g vitamin B 12 (Sigma, V2876) dissolved in water by oral gavage on day 6 after the start of doxycycline treatment, and blood samples were taken by submandibular collection just before and 24 h after the bolus

0.5 ml injected into the vagina of the spouse through the prodom during intercourse and synchronously with ejaculation

Other downstream effectors of FOXO1 may also contribute to the regulation of sarcopenia through alternative mechanisms, which requires a more comprehensive phenotypic analysis in future studies

Storkey C, Davies MJ, Pattison DI (2014) Reevaluation of the rate constants for the reaction of hypochlorous acid (HOCl) with cysteine, methionine, and peptide derivatives using a new competition kinetic approach