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When levels are low, oxidative damage can build up over time, and that can lead to muscle soreness, joint pain, or symptoms that mimic autoimmune flare-ups

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49 The second one is safety, which is a critical issue

It's the single largest determinant of whether the protocol works

Immunostaining After organotypic culture or cryosection, the cochlear explants or tissue sections were fixed with 4% PFA, permeabilized with 1% TritonX-100 in PBS, and blocked by incubation in PBT-1 solution (0.1% Triton X-100, 8% donkey serum, 1% bovine serum albumin, and 0.02% sodium azide in PBS) at room temperature for 1 h