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In another study, when oocytes were fertilized using sperm derived from mice knocked-out for DICER or DROSHA, thus showing defective miRNAs/endo-siRNAs biogenesis, a deregulation in the expression of the embryo preimplantation genes was seen (Yuan et al., 2016)

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Therefore, ZIL may adequately dehydrate the cells to prevent intracellular ice formation like other non-cell-permeable cryoprotectants 20,21,22

However, the experimental data exhibits and offset of 6% corresponding to an analgesic effect in the absence of drug administration that cannot be explained by the paradigm of an increase in anionic current