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Finally, the fragments are placed into a bone preparation device that utilizes vacuum ultrasonic high-pressure sterilization, and different solutions (Liquid A: 3% hydrochloric acid, Liquid B: 10% hydrogen peroxide, and Liquid C: 80% ethanol) are used according to the manufacturing method

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Equal loading of proteins was estimated by re-probing the membranes with mouse anti-GAPDH antibodies (1:1000

doi:10.1074/jbc.M109641200 Imai H, Masayasu H, Dewar D, Graham DI, Macrae IM (2001) Ebselen protects both gray model of focal and white matter in a rodent cerebral ischemia

Bioorg Med Chem 25:126131