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The lentiviral particles for CBS OPT and L2HGDH OPT constructs were generated similarly with the packaging system as those for sgRNA but selected with puromycin at a final concentration of 1.5 g/ml

The Kunming mouse acute toxicity test was conducted by intravenous administration of LAAM GSH-CQDs at a dose of 5000 mg kg 1 on day 0 and observed for 2 weeks

Therefore, the concentration of H 2 O 2 in the cell supernatant was further detected using assay kits

These factors constrain direct clinical extrapolation but do not negate the reproducibility of observed anti-inflammatory and antioxidant effects across populations

Direct injections and IV drips work at the same pace because both deliver nutrients through the bloodstream