micro doses of pt141 WJMH :: World Journal Men's Health WO2005117935A1 - Multiple agent therapy

All patients were kept on elimination diet where patients in PPG were given prick positive food and PNG patients were given corn (most allergenic to RA patients) along with rice (not allergenic) in increased amount for 12 days

HiFi polymerase (Kapa Biosystems, #KK2102), FastDigest restriction enzymes (Thermo Fisher Scientific #FD0274) and T4 DNA ligase (Thermo Scientific # K1423) were used for plasmid construction, with the exception of pHT01::rha1/2_WT and pHT01::rha1/2_MUT which were generated using Gibson Assembly MasterMix (NEB, #E2611S)

In the case of ESI-MS analyses under denaturing conditions, buffer-exchanged proteins were denatured before ESI-MS analyses by adding aliquots of formic acid at final concentration of 30%)

It aids in identifying any worsening of anemia or potential complications, guiding appropriate interventions and adjusting the treatment plan accordingly

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