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To analyze cell dynamics in punch-passaged cultures and compare it with epithelioids generated from explants or single-cell suspensions, six punch-passaged cultures were generated from one membrane portion (cut as explained earlier) from three different mice and treated for 2 weeks in mFAD after confluence

Fortunately, a growing number of recent and rigorous preclinical studies have begun resolving the third challenge, which may also lead to overcoming the first and second barriers