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Primary antibodies included SOD1 (Cu/Zn SOD) (Enzo Life Sciences ADI-SOD-101, 1:1000), SOD2 (Mn/SOD) (Enzo Life Sciences ADI-SOD-111, 1:8000), and -actin (Sigma-Aldrich A5441, 1:5,000) with secondary antibodies Goat-anti-rabbit HRP-conjugated IgG, (Santa-Cruz sc2004) (1:6,000 for SOD1, 1: 8,000 for SOD2) and Goat-anti-mouse HRP-conjugated IgG (Santa-Cruz sc 2005) (1:10,000 for -actin)