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Serum lipopolysaccharide (LPS), interleukins (i.e., IL-2, IL-6, IL-8), tumor necrosis factor-alpha, and cardiac troponin were measured over the course of the study The MVD-dogs were divided into Group 1 (Stage B1 dogs) and Group 2 (Stages B2 and C dogs)

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2.5.2 cDNA synthesis and RT-qPCR Reverse transcription was conducted with 40 ng of RNA per sample using the Reverse Transcription Master Mix Kit (Fluidigm Corporation, San Francisco, California, USA), following the manufacturers instructions

Many people find that regular supplementation reduces irritability and improves stress tolerance