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Western blot analysis RIPA Lysis Buffer (TransGen, Beijing, China) was used to extract total protein from tissues/fibroblasts and a Nuclear and Cytoplasmic Protein Extraction Kit (TransGen) was used to extract the protein from nucleus and cytoplasm of fibroblasts

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Use of Chelation Agents: If a subject is undergoing chelation therapy to remove heavy metals from the body, using GHK-Cu is counterproductive and potentially confusing for the system