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To avoid oxidation of PQH 2 during acetone extraction, the sample was incubated with the solvent for no longer than 2 min, then centrifuged (5 min, 9000 g), evaporated in a stream of nitrogen, dissolved in 200 L of methanol, and injected into an HPLC system

By working smarter, not harder, melatonin can decrease the availabilityof PUFA and therefore cut off the fuel for ROS production and lipid peroxidation