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Metformin Radiosensitizes p53-Deficient Colorectal Cancer Cells through Induction of G2/M Arrest and Inhibition of DNA Repair Proteins

For in vitro binding of mSIN with RICTOR, equal amounts of the purified GST or GST-mSIN proteins immobilized to glutathione sepharose TM 4B beads were incubated with purified SFB-RICTOR either in the presence of recombinant His-PTPN22 or equal volume of corresponding buffer for 1 h at 4 C