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When using liposomes containing cationic lipids, the dried lipid film is resuspended in an appropriate solution such as sterile water or an isotonic buffer solution such as 10 mM Tris/NaCl, sonicated, and then the preformed liposomes are mixed directly with the DNA

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hGH indirectly controls growth and protein synthesis by stimulating the liver and other tissues to produce a group of proteins called insulin-like growth factors (IGFs)

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