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Riordan first became aware of this therapy in the early 1980s, when a 70-year old patient suffering from metastatic renal cell carcinoma that had spread to his liver and lungs came to the clinic requesting IV ascorbate infusions

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These tests can identify the presence of various substances, including SARMs, depending on the sensitivity of the testing method used

The genes were amplified by PCR and inserted into the ligation independent cloning vector (LIC) pLIC-His using the primers shown in Table S2