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This immediate source of glutathione is considered a first line defence against oxidative stress-induced damage 12

For low glucose experiments cells were plated in Neurobasal-A Medium, no D-glucose, no sodium pyruvate, supplemented with 0,5 mg/ml D-Glucose, 1 mM sodium pyruvate (ThermoFisher), and the components previously described for complete NB media, and incubated at 37 and 5% CO 2 for 72 h

Additionally, we previously discovered that the transcriptional mediator complex contributes to antioxidant gene expression via a direct interaction between Nrf2 and a mediator subunit, MED16 (Figure 2) [31]