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A variety of single-cell epigenome-sequencing techniques have been developed, such as single-cell sodium bisulfite sequencing for DNA methylation detection, 345 single-cell chromatin immunoprecipitation sequencing for the identification of histone modification and proteinDNA interaction, single-cell transposable accessible chromatin sequencing, and Hi-C sequencing for the evaluation of chromatin accessibility and chromosome conformational information

Supervised differential gene expression analysis and gene ontology comparison between the combination and control groups revealed an upregulation of pathways associated with autophagy in the combination group for both models (Fig

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