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The activity of class I, II, III and IV of alcohol dehydrogenase (ADH) Isoenzymes and aldehyde dehydrogenase (ALDH) in brain Cancer

doi: 10.1523/JNEUROSCI.19-12-04867.1999

Fig 1B)

Briefly, 1 mL of assay mixture contains optimized concentrations of the following chemicals: 0,5 M K 2 HPO 4 (pH 7.0), 2,5 mM EDTA, 0,18 U/mL GR, 100 mM glutathione and 10 mM reduced NADPH and tissue extract (0,5 mL) was added in the spectrophotometer cuvette along with 0,1 mL of 60 mM cumene hydroperoxide, a suitable substrate for GP