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The assessment of free testosterone and DHEAS concentrations was performed by a commercial enzyme-linked immunosorbent assay) ELISA) kit (Abbott Healthcare Pvt

Therefore, the need for normal breed-specific reference ranges has been emphasised for comparison of pathological changes caused by cardiac disorders." (9) Jacobson, J.H., et al., An echocardiographic study of healthy Border Collies with normal reference ranges for the breed