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Zymography Zymographic analysis was performed on the purified KLIKK proteases, mixed 1:1 with sample buffer (0.125 M Tris-HCl, pH 7.8, 20% glycerol, 4% SDS, and 0.1% Bromophenol Blue) for 15 min at 20C, and then electrophoretically resolved in 12% SDS-PAGE gels (acrylamide/bis-acrylamide ratio, 27.5:1) containing casein or gelatin at a final concentration of 0.1 mg ml 1

p21 does have an apparent role in adipocyte maintenance, however, outside of adipogenic effects not much is known about p21 in regulating lipid metabolism

Cold Spring Harbor Laboratory, pp 309315 Kalaria RN, Harik SI (1989) Reduced glucose transporter at blood-brain barrier and in cerebral cortex in Alzheimers disease

Clin Chim Acta 411:17281734 Wen T, Zhu W, Xue C et al (2014) Novel electrochemical sensing platform based on magnetic field-induced self-assembly of Fe 3 O 4 @polyaniline nanoparticles for clinical detection of creatinine