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H 2 S and pyruvate production with fluorescent probe detection Kinetic H 2 S production was measured using AzMC (Sigma #802409), which is a fluorogenic probe selectively reduced in the presence of H 2 S producing 7-amino-4-methylcoumarin 29 and detectable with excitation wavelength Exc 350 nm and emission wavelength Emi 445 nm

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