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Cell culture and STR analysis ARPE-19 human RPE cells were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA), and cultured in DMEM/F12 medium supplemented with 10% fetal bovine serum at 37 C in air containing 5% CO 2

Vitamin B12 is essential for many bodily functions, and a deficiency can lead to a range of symptoms, some of which can be quite severe if left untreated

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For the spA competition ELISA, a 5-molar excess of spA (Sigma-Aldrich) was allowed to bind captured NIP IgG1 variants before addition of pure C1q

225 - 228 Journal articles | 1999 Gooding JJ, 1999, 'A simple model for an amperometric channel biosensor', Electrochemistry Communications , 1, pp