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Haemoglobin-bound iron is transported into the intestinal epithelial cells by heme carrier protein 1 (HCP1) that is localised to the intestinal mucosal epithelium

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The supernatant was transferred to a new tube

C., Kanter, R

Cell culture and drug treatment The human liver cancer cell line HepG2 was obtained from ATCC and was cultured in DMEM glutamax high glucose medium (Invitrogen) supplemented with 10% fetal bovine serum and 100 U/mL penicillin/streptomycin (Invitrogen), in a humidified incubator at 37C and 5% CO 2 atmosphere