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doi: 10.3389/fendo.2025.1613334 Received 17 April 2025 Accepted 15 August 2025 Published 02 October 2025 Volume 16 - 2025 Edited by Hong Liu, UC Davis Health, United States Reviewed by Andrei Adrian Tica, University of Medicine and Pharmacy of Craiova, Romania Tian Xia, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, China Updates Copyright 2025 Ou, Wang, Zhou, Chen, Lyu, Feng and Huang

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The crRNA sequence must be followed by a short DNA sequence of 26 bp, known as the protospacer adjacent motif (PAM), which is important for Cas9 compatibility