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All experiments were conducted in compliance with relevant guidelines and regulations approved by the Institutional Animal Care and Use Committee (ICAUC) at the University of Kansas Medical Center

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In addition to directly obtaining TMAO from fish and seafood, it is more commonly produced by the gut microbiota through the conversion of foods rich in choline, L-carnitine, and ergothioneine (e.g., red meat, eggs, and mushrooms) (164)