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Subsequently, they observed a significant increase in serum alanine aminotransferase and lipopolysaccharide levels, along with the presence of liver steatosis

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There was significantly higher NeuN+ and FG+ cell density in the GCL of ALA treated mice (p = 0.045, p = 0.047, respectively), suggesting RGC structural and functional protection with dietary ALA

For western blot analysis, cells were collected after 72 h of transfection and lysed in RIPA buffer with protease inhibitor (Roche) and phosSTOP (Roche)