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ApaI, BsmI, FokI and TaqI polymorphisms in the vitamin D receptor (VDR) gene and the risk of psoriasis: a meta-analysis

The slides were immersed in pre-chilled lysis buffer (2.5 M NaCl, 100 mM Na 2 EDTA, 10 mM Tris, 0.2 mM NaOH [pH 10], and Triton X-100) and incubated overnight at 4 C in order to lyse the cells and to permit DNA unwinding