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A: In vitro studies involve cells in a controlled environment, using molar concentrations (M), while in vivo studies involve whole organisms and use weight-based dosages (mg/kg)

The primary downstream pathway involves JAK2 trans-phosphorylation and subsequent activation of STAT5a/b, which drives transcription of key targets including hepatic IGF-1, IGFALS, and feedback regulators CISH and SOCS2

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