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A.Abdel-RahmanM

Cell pellets were washed in DPBS with Ca 2+ , Mg 2+ , centrifugated at 51 g for 5 min and resuspended in Williams E medium supplemented with 10% FBS (Merck), 15 mM HEPES, 1 mM sodium pyruvate, 1% penicillin/streptomycin, 1% MEM NEAA (all provided by Gibco), 1 g ml 1 dexamethasone (Jenapharm) and 32 U l 1 human insulin (Eli Lilly)

Practically, owners can watch for patterns around digestion: does the dogs energy dip coincide with soft stool, frequent bowel movements, or a sudden switch in food