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The 10 l of enzymatic extract was mixed with 90 l of solution consisting of 5 mM guaiacol and 2.5 mM H 2 O 2 in 50 mM acetic buffer (pH 5.6.) Activity measurement was assayed at 37 C in a Nunc U-bottom 96-well plate on a Varioskan LUX Multimode Microplate Reader

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